RBC Real-Time PCR
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RBC-FluoGene® – The high-quality Real-time typing system
RBC-FluoGene® combines minimal hands-on time with reliable real-time PCR.
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The advantages of the FluoGene® system
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Flyer RBC-FluoGene®
The next generation: RBC-FluoGeneNX
RBC FluoGene® ABO
The RBC-FluoGene® ABO basic system is able to clearly distinguish the alleles ABO*A1.01, ABO*A2.01, ABO*B.01, ABO*O.01 & ABO*O.02 from each other. The RBC-FluoGeneNX ABO plus test system also detects another 35 ABO variants, in particular ABO*AW and ABO*BW alleles and impresses with a faster PCR program (54 min) and lower DNA consumption.
RBC FluoGene® CDE
This test system detects the RHCE alleles RHCE*C, *c, *E, *e, as well as the SNP c.122A>G (CW), and SNP c.733C>G. Based on the exon pattern via detection of RHD exons 1-7, as well as 9, and 10, a variety of RHD categories and hybrid genes are uniquely characterized. In addition, RHD null alleles and further partial Ds (separation of the DAU allele cluster) are identified via specific SNPs.
RBC FluoGene® CDE eXtend
The CDE eXtend Kit is based on the CDE Kit and enables a comprehensive RHD and RHCE genotyping by additional reactions. In addition to further partial D, many weak D and DEL alleles are also detected. Furthermore, additional RHCE SNPs are integrated for the detection of RHCE variants.
RBC FluoGene D® weak/variant
The molecular characteristics of the weak D types are different point mutations, each causing an amino acid exchange in the intracellular or transmembrane region of the affected antigen. The resulting lower concentration of Rh/D epitopes on the erythrocyte membrane depends on the weak D type presence and can lead to a strong attenuation of agglutination in the classical hemagglutination test. The RBC FluoGene D weak/variant detects the most common weak D alleles (type 1, 1.1, 2, 3, 4, 4.0, 4.1, 4.2, 4.3, 5, 15 and 17), as well as the DEL alleles RHD*01EL.01, and RHD*01EL.08.
RBC FluoGene® vERYfy
This test system combines the typing of different blood group systems in parallel. Serologically detected D-ambiguities, unclear blood groups of polytransfused patients or patients producing allo- or auto-antibodies and/or showing a positive DAT test can be screened and identified with this kit.
In the Rhesus system, exons 3, 5, and 10, as well as the RHD pseudogene ψ (37 bp insertion in intron 3) are detected within the RHD gene, as well as the RHCE alleles RHCE*C, *c, *E, *e, and *Cw. In addition, this kit detects the blood group systems Kell (KEL*01.01, KEL*02), Kidd (JK*01/JK*02), Duffy with the alleles FY*01, FY*01, FY*02W.01, and FY*02N.01 (GATA-box mutation), MNS with the alleles GYPA*01(M), GYPA*02(N), GYPB*03(S), GYPB*04(s), U+var(P2), and U+var(NY), and Dombrock (DO*01/DO*02).
RBC FluoGene vERYfy® eXtend
RBC FluoGene vERYfy® eXtend
RBC-FluoGene® vERYfy eXtend was developed for advanced blood group typing and is a combination of the inno-train blood group typing kits. With only one test the characteristics of RHD/RHCE, KEL, JK, FY, MNS, DO, LU, YT, DI, CO, KN, and LW can be examined. For more information, please refer to the specificity table enclosed in the kit and the table below.
RBC-FluoGene® Rare
The present detection system allows the typing of the rare blood group alleles Diego (DI*01/DI*02), Wright (DI*02.03/DI*02. 04), Cartwright (YT*01/YT*02), Lutheran (LU*01/LU*02), Kell (KEL*02.03 and KEL*02.06), Colton (CO*01.01/CO*02.01), Knops (KN*01/KN*02), and Landsteiner-Wiener (LW*05/LW*07). The blood group systems mentioned are biallelic with one common and one rare allele each.
RBC-FluoGene® D-Screen
The test system allows the detection of RHD exons 3, 5, and 10 in only one reaction.
The screening test is designed for molecular confirmation of serologically negative RHD donor samples. In case of positive screening reactions, the samples should be further differentiated.
Products:
RBC FluoGene Kits
Item number | Name | Test / Plate |
---|---|---|
RUO | Not for use in diagnostic procedures- USA and Canada | |
001 081 010 | RBC-FluoGene vERYfy | 1 |
001 081 030 | RBC-FluoGene vERYfy | 3 |
001 082 010 | RBC-FluoGene CDE | 1 |
001 082 040 | RBC-FluoGene CDE | 4 |
001 083 010 | RBC-FluoGene D weak/variant | 1 |
001 083 040 | RBC-FluoGene D weak/variant | 4 |
001 085 010 | RBC-FluoGene Rare | 1 |
001 086 010 | RBC-FluoGene ABO basic | 1 |
001 086 040 | RBC-FluoGene ABO basic | 4 |
001 087 048 | RBC-FluoGene D screen | 12 |
001 087 240 | RBC-FluoGene D-Screen XT | 24 |
001 088 048 | RBC-FluoGene Vel-Screen | 12 |
001 089 010 | RBC-FluoGene KKD | 1 |
001 091 010 | RBC-FluoGene vERYfy eXtend | 1 |
001 092 010 | RBC-FluoGene CDE eXtend | 1 |
RBC FluoGene NX Kits
Item number | Name |
---|---|
001 080 010 | RBC-FluoGene NX ABO Plus |
Licensor Dyes and Quenchers
Elitech Dyes and Quenchers
“EclipseTM Dark Quencher”, “EclipseTM Quencher” are trademarks of ELITechGroup, Inc., “Yakima Yellow®”, “AquaPhluor®”, “Redmond Red®” and “Eclipse®” are registered trademarks of ELITechGroup, Inc.
Use of this product (including but not limited to Yakima Yellow® and EclipseTM Dark Quencher) is covered by patents owned by ELITechGroup, Inc., and sublicensed by Kaneka Eurogentec S.A. Such patents include without limitation the following US patents and corresponding patent claims outside the US: US6,699,975, US 6,790,945, US 7,662,942, US 6,653,473, US 6,972,339, 7,541,454, 7,671,218, 7,767,834, 8,163,910, 6,727,356. Further information on purchasing licences may be obtained by contacting licensing@eurogentec.com.
Downloads
Flyer FluoGene®
Flyer FluoGene® Kits